Drosophila genital morphometric analysis (Drosophilidae)

Knowledge graph centered on Drosophila genital morphometric analysis (Drosophilidae) with 19 nodes and 45 connections. Top connected: frequency-dependent selection, The extent of variation in male song, wing and gen, Drosophila melanogaster, ecological speciation, Melospiza melodia.

Method synopsis

Chemical clearing of Drosophila male genitalia using NaOH treatment, followed by microscopic imaging and principal component analysis of genital shape variation.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — The extent of variation in male song, wing and genital characters among allopatric <i>Drosophila montana</i> populations (2007), Journal Compilation of European Society for Evolutionary Biology. Implementations in other papers (listed below) may differ.

1. Genital preparation and analysis
   Genitalia removed from male bodies preserved in 70% ethanol, transferred singly into Eppendorf tubes with 0.1 M NaOH and kept at 95°C for 8 min. NaOH replaced with water, distiphallus removed and placed on glass slide with fine forceps.
   Quantities: Individual genitalia processed separatelyDuration: 8 minutes at 95°CConditions: 95°C heating, fine manipulation under microscope
   Equipment: Eppendorf tubes, 0.1 M NaOH, glass slides, fine forceps, EUKRIDT microscope, SPOT Insight colour digital camera, Zeiss Axioscan 40 microscope

Typical Equipment

• Eppendorf tubes
• 0.1 M NaOH
• EUKRIDT microscope
• SPOT Insight colour digital camera
• SHAPE 1.2 program

Output Measurements

• genital principal component scores
• genital hook length
• genital size

Papers Using This Protocol (1)

Species Studied With This Protocol