diallel mating design (Polemoniaceae)

Knowledge graph centered on diallel mating design (Polemoniaceae) with 44 nodes and 152 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Scarlet Gilia, Slender-tube Skyrocket, Crested Butte.

Method synopsis

Production of F2 hybrid generations with alternate cytoplasmic backgrounds through controlled hand-pollination crosses. Allows testing of cytoplasmic effects on physiological traits.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Leaf physiology reflects environmental differences and cytoplasmic background in <i>Ipomopsis</i> hybrids (2007), American Journal of Botany. Implementations in other papers (listed below) may differ.

1. Produce experimental F2 hybrids
   Generated reciprocal F2 hybrids through hand-pollination crosses. F1 hybrids with alternate cytoplasmic backgrounds maintained in pots in RMBL garden, then reciprocally hand-pollinated to produce F2 generation with known cytoplasmic backgrounds.
   Quantities: F2T (I. tenuifuba cytoplasm) N=100, F2A (I. aggregata cytoplasm) N=233, total 2630 seeds plantedDuration: Multi-generational crossing program over multiple growing seasonsConditions: Common garden conditions at Rocky Mountain Biological Laboratory
   Equipment: hand-pollination tools, greenhouse pots, WeatherPort portable mesh greenhouses

Typical Equipment

• hand-pollination tools
• greenhouse pots
• WeatherPort portable mesh greenhouses

Output Measurements

• F2 hybrids with known cytoplasmic backgrounds

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used