Egg mass density manipulation experiment (Baetidae)

Knowledge graph centered on Egg mass density manipulation experiment (Baetidae) with 39 nodes and 137 connections. Top connected: Lepidoptera, Hydrologic and behavioral constraints on ovipositi, aquatic insects, Ambystoma, Baetis.

Method synopsis

Experimental manipulation of existing egg mass densities on preferred substrates to test whether females exhibit aggregative oviposition behavior in response to conspecific egg presence.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Selective oviposition by the mayfly <i>Baetis bicaudatus</i> (2006), Oecologia. The protocol was originally introduced by Hydrologic and behavioral constraints on oviposition of stream insects: implications for adult dispersal (2000), Oecologia. Implementations in other papers (listed below) may differ.

1. Conduct aggregation experiment
   Selected 15 rocks that had at least ten egg masses and characterized as 'good' oviposition sites. Using maps of egg mass locations, counted new egg masses for 4 consecutive days then assigned rocks randomly to three treatments: (1) removed all egg masses except one, (2) removed all egg masses except 20, (3) removed all egg masses except 50. Counted new egg masses daily for next 4 days and removed new masses to re-establish treatments.
   Quantities: 15 rocks with >10 egg masses, treatments of 1, 20, and 50 remaining egg masses, monitored for 4 days before and 4 days after treatmentDuration: July 2003, 4 days pre-treatment monitoring plus 4 days post-treatment monitoring with daily countsConditions: Fast current velocity locations (>500 cm²), B. bicaudatus flight period

Output Measurements

• daily new egg mass counts
• aggregation response measures

Papers Using This Protocol (2)

Species Studied With This Protocol