Knowledge graph centered on COI DNA barcoding (Baetidae) with 35 nodes and 127 connections. Top connected: Rushes, aquatic insects, Ambystoma, phenotypic plasticity, Ambystoma tigrinum.
DNA extraction using modified Chelex protocols followed by PCR amplification and sequencing of the mitochondrial cytochrome oxidase I gene to assess genetic variation within and among mayfly populations.
Synthesized from method descriptions across 2 papers using this protocol.
Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Are populations of mayflies living in adjacent fish and fishless streams genetically differentiated? (2005), Freshwater Biology. Implementations in other papers (listed below) may differ.