Electrolyte leakage assay (Asteraceae)

Knowledge graph centered on Electrolyte leakage assay (Asteraceae) with 59 nodes and 129 connections. Top connected: Rocky Mountain Biological Laboratory, Engelmann Spruce, Asteraceae, Potentilla gracilis, Potentilla pulcherrima.

Method synopsis

Controlled temperature treatments from 15-65°C applied to detached leaves for 1 hour, with temperature ramped at 2°C/hour. Used to determine lethal temperature thresholds via electrolyte leakage measurements.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — High-temperature tolerance of <i>Artemisia tridentata</i> and <i>Potentilla gracilis</i> under a climate change manipulation (1996), Oecologia. Implementations in other papers (listed below) may differ.

1. Measure leaf temperatures
   Control and heated plots were measured with an Everest Interscience model 210 infrared thermometer held 8 cm from the leaves. Terminal leaves were removed from each plant with scissors and immediately placed with petioles in water inside 30-ml capped plastic bottles for transport.
   Quantities: Measurements from tagged plants in all plotsDuration: 0600-0630 hours collection timeConditions: Early morning collection to minimize stress
   Equipment: Everest Interscience model 210 infrared thermometer, scissors, 30-ml capped plastic bottles
2. Conduct temperature treatments
   High-temperature treatments were conducted within a modified 0.18 × 0.26 × 0.18 m picnic cooler. Temperature was maintained by a 0.18 × 0.18 m heat exchanger within the picnic cooler. Petioles were re-cut under water by small pruning shears and the leaf blades placed in the heat exchanger. Treatments started at ambient temperature (5-10°C) and increased at 2°C/h to desired temperature. Leaves were treated for 1 h at temperatures ranging from 15°C to 65°C.
   Quantities: Temperature range 15-65°C, 2°C/h heating rate, 1 hour treatment durationDuration: 1 hour treatment time at target temperature, plus heating timeConditions: Controlled temperature environment, humidity maintained at saturation vapor pressure
   Equipment: modified picnic cooler (0.18 × 0.26 × 0.18 m), heat exchanger (0.18 × 0.18 m), circulating water bath, small pruning shears

Typical Equipment

• modified picnic cooler
• heat exchanger
• circulating water bath
• 60% ethylene glycol solution
• number 1 cork borer
• Cole-Parmer Chemcadet pH/conductivity meter

Output Measurements

• LT50 values
• electrolyte leakage percentages

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used