Knowledge graph centered on Controlled freezing stress treatment (Liliaceae) with 30 nodes and 107 connections. Top connected: Delphinium, Asteraceae, Potentilla, Erigeron, Delphinium barbeyi.
In situ freezing treatment using liquid nitrogen and heat exchanger to gradually cool plant tissues to -30°C. Designed to simulate natural freezing events while allowing controlled temperature ramping and monitoring.
Synthesized from method descriptions across 1 paper using this protocol.
Steps below were extracted from the paper that introduces this protocol — <i>In situ</i> photosynthetic freezing tolerance for plants exposed to a global warming manipulation in the Rocky Mountains, Colorado, USA (2004), New Phytologist. Implementations in other papers (listed below) may differ.