Knowledge graph centered on Spatial array microcosm experiment with 15 nodes and 27 connections. Top connected: Bacteria, Paramecium bursaria, University of Calgary, Calgary, Protist population monitoring.
Method synopsis
Laboratory experiment using interconnected bottle arrays to manipulate spatial heterogeneity in protist food webs and test effects on species coexistence.
Synthesized from method descriptions across 1 paper using this protocol.
Build 4x4 arrays of 16 interlinked bottles using Tygon tubing connections between bottles arranged on the diagonal
Quantities: 16 bottles per array, 3 arrays total (2 HO, 1 HE)Duration: Initial setup before experimentConditions: Ambient light and temperature in laboratory
Equipment: bottles, Tygon tubing
Prepare culture media
Add 800 ml of nutrient medium (one protozoan pellet per litre of deionized water) to each array, with each bottle containing 50 ml of protozoan media
Quantities: 800 ml total per array, 50 ml per bottleDuration: Initial setupConditions: Laboratory conditions
Equipment: protozoan pellets, deionized water
Implement spatial treatments
For HO treatment: remove half the volume of each bottle every second day, mix water together, redistribute over entire array. For HE treatment: leave array unmixed for natural dispersal through tube connections
Quantities: 50% of bottle volume (400 ml total) removed and redistributed in HODuration: Every second day for 25 daysConditions: Laboratory conditions