Protist population monitoring

Knowledge graph centered on Protist population monitoring with 15 nodes and 27 connections. Top connected: Bacteria, Paramecium bursaria, University of Calgary, Calgary, Spatial array microcosm experiment.

Method synopsis

Regular sampling and counting of protist populations in microcosm bottles using Lugol's fixation and microscopic identification.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Species coexistence in a food web with intraguild predation (2004), URBEE. Implementations in other papers (listed below) may differ.

1. Monitor populations
   Remove 0.5 ml sample from each bottle, gently mix before sampling, fix with Lugol's solution, identify and count protists. For HE arrays, place clips on Tygon connections to prevent movement during sampling
   Quantities: 0.5 ml sample per bottle, replaced with 0.5 ml autoclaved mediaDuration: Every 2 days from day 1 to day 25Conditions: Laboratory conditions
   Equipment: Lugol's solution, clips, autoclaved media

Typical Equipment

• Lugol's solution
• clips

Output Measurements

• population density
• species abundance

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used