Butterfly wing RNAi electroporation (Nymphalidae)

Knowledge graph centered on Butterfly wing RNAi electroporation (Nymphalidae) with 24 nodes and 68 connections. Top connected: Beetles, Lepidoptera, Speyeria mormonia, Speyeria, Insecta.

Method synopsis

Electroporation of dsRNA into developing pupal wings to knock down gene expression and test for homeotic transformations of scale types

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Genetic basis of an adaptive polymorphism controlling butterfly silver iridescence (2025), Current Biology. Implementations in other papers (listed below) may differ.

1. RNAi electroporation
   Dissected pupal wings and electroporated with optix dsRNA using BTX ECM 830 electroporator. Applied 5 square pulses of 280 ms at 10 V, separated by 100-ms intervals to dorsal pupal wings
   Quantities: 5 pulses, 280 ms duration, 10 V, 100-ms intervalsDuration: Electroporation procedureConditions: Pupal stage wings at 28% and 54% development
   Equipment: BTX ECM 830 electroporator, phosphate buffered saline, Custom DsiRNAs

Typical Equipment

• BTX ECM 830 electroporator
• Custom DsiRNAs

Output Measurements

• scale color transformations
• gene expression knockdown

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used