Wald test

Knowledge graph centered on Wald test with 14 nodes and 38 connections. Top connected: Crested Butte, Boechera stricta, Bacteria, quadrat sampling method, Archaea.

Method synopsis

Statistical analysis using Wald test to identify taxa with significant abundance changes between treatment and control conditions, with Benjamini-Hochberg correction for multiple testing.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — The impacts of environmental change on plant and microbial communities: A turf transplant experiment in the Colorado Rocky Mountains (2025). Implementations in other papers (listed below) may differ.

1. Establish study sites
   Established study sites at High (3300 m, 38.97274 N, -107.04908 W), Mid (3150 m, 38.96046 N, -107.03262 W), and Low (2900 m, 38.93248 N, -107.01018 W) elevation in the subalpine meadows of the Elk Mountains near Crested Butte, Colorado, USA. Each site location had a southern aspect and approximate 10% slope.
   Quantities: 3 sites across 400-meter elevation gradientDuration: Established in 2017Conditions: Subalpine meadows with southern aspect and 10% slope
   Equipment: GPS coordinates measurement tools
2. Layout turf plots
   Each site was divided into five blocks of five 0.5 m x 0.5 m turfs arranged perpendicularly to the hillslope. Focused on grassland (grass/forb) vegetation and excluded shrubs when selecting turfs. Treatments were assigned randomly within each block.
   Quantities: 25 turfs per site (5 blocks × 5 turfs), total of 75 turfs initially, expanded to 90 turfs in 2018 with addition of sixth blockDuration: Initial setup in 2017, expanded in 2018Conditions: Grassland vegetation areas, avoiding shrubs
   Equipment: measurement tools for 0.5m x 0.5m plot demarcation

Typical Equipment

• Wald test statistical analysis
• Benjamini-Hochberg statistical method

Output Measurements

• logarithmic fold change
• p-values
• significant taxa

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used