Wald test

Knowledge graph centered on Wald test with 21 nodes and 89 connections. Top connected: Crested Butte, Boechera stricta, Bacteria, phenological mismatch, Climate Change Impacts.

Method synopsis

Statistical analysis using Wald test to identify taxa with significant abundance changes between treatment and control conditions, with Benjamini-Hochberg correction for multiple testing.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — The impacts of environmental change on plant and microbial communities: A turf transplant experiment in the Colorado Rocky Mountains (2025). Implementations in other papers (listed below) may differ.

1. Establish study sites
   Established study sites at High (3300 m, 38.97274 N, -107.04908 W), Mid (3150 m, 38.96046 N, -107.03262 W), and Low (2900 m, 38.93248 N, -107.01018 W) elevation in the subalpine meadows of the Elk Mountains near Crested Butte, Colorado, USA. Each site location had a southern aspect and approximate 10% slope.
   Quantities: 3 sites across 400-meter elevation gradientDuration: Established in 2017Conditions: Subalpine meadows with southern aspect and 10% slope
   Equipment: GPS coordinates measurement tools
2. Layout turf plots
   Each site was divided into five blocks of five 0.5 m x 0.5 m turfs arranged perpendicularly to the hillslope. Focused on grassland (grass/forb) vegetation and excluded shrubs when selecting turfs. Treatments were assigned randomly within each block.
   Quantities: 25 turfs per site (5 blocks × 5 turfs), total of 75 turfs initially, expanded to 90 turfs in 2018 with addition of sixth blockDuration: Initial setup in 2017, expanded in 2018Conditions: Grassland vegetation areas, avoiding shrubs
   Equipment: measurement tools for 0.5m x 0.5m plot demarcation

Typical Equipment

• Wald test statistical analysis
• Benjamini-Hochberg statistical method

Output Measurements

• logarithmic fold change
• p-values
• significant taxa

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used