Bumble bee honey ethanol quantification (Apidae)

Knowledge graph centered on Bumble bee honey ethanol quantification (Apidae) with 51 nodes and 149 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee.

Method synopsis

A laboratory protocol for extracting honey samples from captive bumble bee colonies and quantifying ethanol content using enzyme-based assays. Samples are collected over time from maintained colonies to assess natural ethanol accumulation.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• 200 μL pipette
• 1.5 mL microcentrifuge tubes
• qPCR-grade water
• parafilm
• freezer at -80°C
• balance for weighing
• handheld refractometer
• enzyme-based ethanol assay kit
• 96-well plates
• plate reader

Output Measurements

• honey ethanol concentration
• total sugar concentration
• honey mass

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used