Synthetic nectar volatile collection and GC-MS analysis (Fungi)

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Method synopsis

Method for collecting and analyzing volatile organic compounds from yeast-inoculated synthetic nectar using headspace sampling with scent traps and gas chromatography-mass spectrometry identification.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• 22 mL vials
• PTFE septum
• Carboxen (40-60 mesh)
• Tenax TA (60-80 mesh)
• micro air sampler Supelco PAS-500
• GC-MS
• Markes autoloading system

Output Measurements

• volatile compound identity
• peak area
• mass spectra

Papers Using This Protocol (1)

Species Studied With This Protocol