quantitative PCR (Brassicaceae)

Knowledge graph centered on quantitative PCR (Brassicaceae) with 24 nodes and 95 connections. Top connected: Rocky Mountain Biological Laboratory, Crested Butte, Boechera stricta, Bacteria, native plants.

Method synopsis

Quantitative PCR using bacterial-specific primers to measure bacterial load in leaf tissue samples across plant patches

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Insect herbivory reshapes a native leaf microbiome (2020), Nature Ecology & Evolution. Implementations in other papers (listed below) may differ.

1. Establish field survey plots
   Set up replicate 0.5 m² plots of native bittercress along transects in sub-alpine and alpine streams. Plots were established to survey herbivore damage from the specialist leaf-mining insect Scaptomyza nigrita.
   Quantities: 24 plots at Emerald Lake site, 60 plots at North Pole Basin site, totaling 84 plots across two sitesDuration: Established once per year for two field seasons (2012, 2013)Conditions: Sub-alpine and alpine stream environments near Rocky Mountain Biological Laboratory
   Equipment: 0.5 m² sampling frames

Typical Equipment

• qPCR system
• bacterial-specific primers

Output Measurements

• bacterial load per leaf
• copies per gram tissue

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used