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Knowledge graph centered on Acute restraint stress protocol (Ambystomatidae) with 19 nodes and 69 connections. Top connected: Ambystoma, Ambystoma tigrinum, Ambystoma tigrinum nebulosm, tiger salamander, interspecific competition.
Standardized protocol for inducing acute stress in amphibians through manual restraint to measure stress hormone response over time. Includes baseline sampling followed by stress induction and temporal monitoring of stress hormone levels.
Synthesized from method descriptions across 1 paper using this protocol.