Diatom transplantation and nutrient exposure experiment (Protista)

Knowledge graph centered on Diatom transplantation and nutrient exposure experiment (Protista) with 10 nodes and 25 connections. Top connected: nutrient limitation, Didymosphenia geminata, algae bloom, Blooms of benthic diatoms in phosphorus-poor strea, stalk production.

Method synopsis

Controlled exposure of D. geminata cells to different water sources with varying nutrient levels to test cellular division and mat formation responses. Involves transplanting cells between environments and monitoring cellular behavior over time.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure as described in the canonical source

Steps below were extracted from the paper that introduces this protocol — Blooms of benthic diatoms in phosphorus-poor streams (2017), Frontiers in Ecology and the Environment. Implementations in other papers (listed below) may differ.

1. Transplant D. geminata into different water sources
   D. geminata cells were transplanted from existing populations into spring-fed creeks that were tributaries of rivers already infested with D. geminata, and also exposed to river water versus spring water in controlled conditions
   Quantities: Not specifiedDuration: 8 days for controlled exposure experimentsConditions: Spring water and river water with different nutrient levels, particularly phosphorus concentrations
   Equipment: Not specified
2. Monitor cellular division rates
   Observed and quantified the percentage of D. geminata cells in late stages of cell division under different phosphorus conditions
   Quantities: 40% of cells in late division stages under spring water, 10% under river waterDuration: 8 daysConditions: Controlled water exposure conditions
   Equipment: Microscopy equipment

Typical Equipment

• Microscopy equipment

Output Measurements

• cellular division rates
• stalk length
• mat formation success

Papers Using This Protocol (1)

Species Studied With This Protocol