Waiting on a mule deer crossing…
Knowledge graph centered on Microbial dormancy molecular analysis with 13 nodes and 43 connections. Top connected: Crested Butte, Bacteria, Washington Gulch, Almont, Judd falls.
DNA and RNA co-extraction followed by cDNA synthesis and next-generation sequencing to assess relative abundance of active versus dormant microbial operational taxonomic units. Uses ratio of rRNA to rDNA recovery to determine dormancy levels.
Synthesized from method descriptions across 1 paper using this protocol.