Microbial dormancy molecular analysis

Knowledge graph centered on Microbial dormancy molecular analysis with 11 nodes and 23 connections. Top connected: Bacteria, elevational gradients, drive-point piezometer groundwater sampling, 16S rRNA gene sequencing, viral operational taxonomic units.

Method synopsis

DNA and RNA co-extraction followed by cDNA synthesis and next-generation sequencing to assess relative abundance of active versus dormant microbial operational taxonomic units. Uses ratio of rRNA to rDNA recovery to determine dormancy levels.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• MoBio DNeasy kits
• RNeasy kits
• random hexamers
• SMARTscribe Reverse Transcriptase Kit
• next-generation sequencing platform

Output Measurements

• relative abundance of active OTUs
• relative abundance of dormant OTUs
• rRNA:rDNA ratios

Papers Using This Protocol (1)

Species Studied With This Protocol