Surface sterilization and isolation of leaf endophytes (Poaceae)

Knowledge graph centered on Surface sterilization and isolation of leaf endophytes (Poaceae) with 16 nodes and 79 connections. Top connected: climate change, Bacteria, species diversity, Festuca, Festuca thurberi.

Method synopsis

Surface sterilization and cultivation of leaf endophytes from grass blade segments using ethanol and sodium hypochlorite sterilization followed by plating on culture medium. Isolates were maintained under controlled temperature conditions.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• 95% ethanol
• 1% sodium hypochlorite
• 70% ethanol
• culture plates
• plating medium

Output Measurements

• culturable fungal isolates
• morphological OTUs

Papers Using This Protocol (1)

Species Studied With This Protocol