Clipping herbivory simulation

Knowledge graph centered on Clipping herbivory simulation with 70 nodes and 152 connections. Top connected: Hummingbird, Bombus, Ipomopsis, Ligusticum porteri, I. aggregata.

Method synopsis

Experimental clipping treatment designed to reduce plant photosynthetic capacity by approximately 50% through removal of stems. Used to simulate herbivory stress and examine effects on mycorrhizal associations.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — When resources don't rescue: flowering phenology and species interactions affect compensation to herbivory in <i>Ipomopsis aggregata</i> (2012), Oikos. The protocol was originally introduced by The effects of ungulate herbivory and nutrient variation on pollen reciept in Ipomopsis aggregata (2002). Implementations in other papers (listed below) may differ.

1. Herbivory simulation through clipping
   Clipped plants at ~2 cm of their base after first observing mule deer actively foraging at sites. All plants were loosely tied to a metal stake to discourage herbivory by deer. Plants tied to stakes were freely visited by pollinators, seed predators and nectar robbers.
   Quantities: Clipped to ~2 cm of base, one clipping event per plantDuration: Clipping performed after herbivory behavior was first observedConditions: Standardized to examine how resource addition and interactions with other species affected plant response to timing of clipping most closely resembling that of natural browsing
   Equipment: clipping tools, metal stakes

Typical Equipment

• clippers
• paper towels
• plastic ziplock bags
• refrigerator
• drying oven

Output Measurements

• dry weight of clipped material
• stress response in mycorrhizal colonization

Papers Using This Protocol (4)

Species Studied With This Protocol