Bacterial endophyte isolation from crucifer leaves (Brassicaceae)

Knowledge graph centered on Bacterial endophyte isolation from crucifer leaves (Brassicaceae) with 17 nodes and 81 connections. Top connected: Ligusticum porteri, Bacteria, Arabidopsis, Arabidopsis thaliana, Cardamine cordifolia.

Method synopsis

Surface sterilization of plant leaves followed by tissue plating on selective media to isolate bacterial endophytes and assess their growth characteristics and pathogenicity.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• cork borer
• Kings B media agar
• 70% ethanol
• blunt syringe
• solid agar plates

Output Measurements

• bacterial colony counts
• growth rate
• virulence symptoms

Papers Using This Protocol (1)

Species Studied With This Protocol