Bumble bee pathogen microscopy survey (Apidae)

Knowledge graph centered on Bumble bee pathogen microscopy survey (Apidae) with 26 nodes and 99 connections. Top connected: Bombus, Bombus terrestris, Bombus appositus, Bombus impatiens, B. appositus.

Method synopsis

Dissection of bumble bees followed by microscopic examination of gut and fat body tissues for protozoan parasites using Giemsa staining. Quantifies pathogen intensity by counting cells in standardized fields of view.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• dissection tools
• microscope slides
• methanol
• Giemsa solution
• phosphate buffer
• water
• compound microscope

Output Measurements

• pathogen presence/absence
• pathogen intensity (cell counts)
• infection prevalence

Papers Using This Protocol (1)

Species Studied With This Protocol