Bumble bee pathogen microscopy survey (Apidae)

Knowledge graph centered on Bumble bee pathogen microscopy survey (Apidae) with 51 nodes and 149 connections. Top connected: Rocky Mountain Biological Laboratory, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee, Buff-tailed Bumble Bee.

Method synopsis

Dissection of bumble bees followed by microscopic examination of gut and fat body tissues for protozoan parasites using Giemsa staining. Quantifies pathogen intensity by counting cells in standardized fields of view.

Synthesized from method descriptions across 1 paper using this protocol.

Typical Equipment

• dissection tools
• microscope slides
• methanol
• Giemsa solution
• phosphate buffer
• water
• compound microscope

Output Measurements

• pathogen presence/absence
• pathogen intensity (cell counts)
• infection prevalence

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used