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Nutritional stress impacts many insect species that have differing reproductive strategies and life histories, yet it is unclear how nutrient-sensing signaling pathways mediate tissue-specific responses to changes in dietary input. In Drosophila melanogaster, insulin/insulin-like growth factor (IIS) and mTOR-mediated signaling within adipocytes regulates oogenesis. To facilitate comparative study of nutrient-sensing pathway activity in the fat body, we developed antibodies to assess IIS (anti-FOXO) and mTOR signaling (anti-TOR) across three µP microPublication Biology Submit a microPublication nymphalid species (Lepidoptera). By optimizing whole-mount fat body immunostaining, we find FOXO nuclear enrichment in adult adipocytes, like that observed in Drosophila. Additionally, we show a previously uncharacterized TOR localization pattern in the fat body. µP microPublication Biology Submit a microPublication
Knowledge graph centered on Antibody development to identify components of IIS with 29 nodes and 122 connections. Top connected: Two-form Bumble Bee, Bumble Bees, Buff-tailed Bumble Bee, Yellow-fronted Bumble Bee, Common Eastern Bumble Bee.
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