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Gut contents of 1,463 stonefly nymphs, comprising 10 species, from the Gunnison River, Colorado, were analyzed from Dec., 1974–Oct., 1975, in relation to food availability. Pteronarcella badia fed primarily on detritus and moss. Perlidae and Perlodidae mature nymphs were all carnivorous, but showed considerable seasonal-developmental shifting in diets and preference during earlier stages. Early instar Isoperla fulva nymphs were herbivore-detritivores, then gradually shifted through an omnivorous to carnivorous diet as development proceeded. Claassenia sabulosa and Hesperoperla pacifica remained carnivorous throughout development. Dominant prey groups were chironomids, mayflies and caddisflies. Horn's Coefficient of Dietary Overlap showed significance among all predator species for major food categories, but subtle mechanisms such as prey species-and size-selectivity and temporal succession provided sufficient partitioning of the abundant food resources to allow for coexistence. Large Claassenia sabulosa nymphs in August selected more mayflies after dark than in the afternoon. No behavioral selection by predacious stoneflies was indicated for the chironomids Ablabesmyia sp., Cricotopus sp., Prodiamesa sp., and Rheotanytarsus sp.
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