factorial experiment (Ranunculaceae)

Knowledge graph centered on factorial experiment (Ranunculaceae) with 70 nodes and 173 connections. Top connected: Hummingbird, Bombus, Ipomopsis, Ligusticum porteri, Delphinium.

Method synopsis

Controlled factorial experiment manipulating both water availability and pollen supplementation to test for interactive effects on plant reproduction.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Does pollination interact with the abiotic environment to affect plant reproduction? (2025), Annals of Botany. The protocol was originally introduced by Does pollination interact with the abiotic environment to affect plant reproduction? (2025), Annals of Botany. Implementations in other papers (listed below) may differ.

1. Establish watering experiment plots
   For D. nuttallianum, established six pairs of plots (12 total) that were 1 m × 1 m in size within same population as study 1. Plot pairs were between 1.25 and 1.5 m away from each other with similar slope and aspect. For H. fendleri, established ten blocks of four individuals (40 total) within same population, randomly assigned to either water addition or control and pollination treatment in factorial combination.
   Quantities: 12 plots for D. nuttallianum (6 pairs), 40 individual H. fendleri plants (10 blocks of 4)Duration: Single growing season (2022)Conditions: Within same populations as study 1
   Equipment: SprayMate Storm 2.5-Gallon 18V Battery Powered Sprayer
2. Apply water addition treatments
   Hand pollinated supplemented plants every Monday, Wednesday and Friday weather permitting. Applied watering using 15 L plastic backpack sprayer consisting of 4 L of water per watering application. Watering occurred from 11 June to 1 July for D. nuttallianum and 15 June to 19 July for H. fendleri during flowering period, stopping after last flower per plot (for D. nuttallianum) or plant (for H. fendleri) was no longer open.
   Quantities: 4 L water per watering event, applied at 16:00 h each day to minimize evapotranspirationDuration: Daily during flowering period (approximately 3 weeks per species)Conditions: After 16:00 h each day to minimize evapotranspiration, weather permitting

Typical Equipment

• SprayMate Storm 2.5-Gallon 18V Battery Powered Sprayer
• 15 L plastic backpack sprayer
• Meter SC-1 porometer

Output Measurements

• fruits per flower
• seeds per fruit
• seeds per plant
• soil moisture
• stomatal conductance

Papers Using This Protocol (4)

Species Studied With This Protocol