soil enzyme activity assay

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Method synopsis

Measurement of six different soil extracellular enzyme activities using microplate spectrophotometry to understand microbial activity and nutrient cycling processes in response to plant removal treatments.

Synthesized from method descriptions across 3 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Soil signals of key mechanisms driving greater protection of organic carbon under aspen compared to spruce forests in a North American montane ecosystem (2025), Biogeosciences. Implementations in other papers (listed below) may differ.

1. Enzyme activity assays
   Measured activity of β-glucosidase and N-acetyl-β-D-glucosaminidase using fluorescence substrate approach. Added 4-methylumbelliferyl β-D-glucopyranoside for BGase and 4-methylumbelliferyl N-acetyl-β-D-glucosaminide for NAGase to soil slurries, incubated, and measured fluorescence using Synergy HT microplate reader.
   Quantities: Enzyme assays on soil samples from multiple depthsDuration: Laboratory incubation and analysis periodsConditions: Controlled laboratory incubation conditions
   Equipment: 4-methylumbelliferyl β-D-glucopyranoside, 4-methylumbelliferyl N-acetyl-β-D-glucosaminide, Synergy HT microplate reader Agilent USA

Typical Equipment

• Synergy HT microplate spectrophotometer
• sodium acetate buffer

Output Measurements

• α-glucosidase activity
• β-glucosidase activity
• cellobiohydrolase activity
• β-Xylosidase activity
• β-N-acetylglucosamini-dase activity
• acid phosphatase activity

Papers Using This Protocol (3)

Species Studied With This Protocol

Places Where Used