MAGIC method

Knowledge graph centered on MAGIC method with 29 nodes and 80 connections. Top connected: Bacteria, Daphnia, mountain hydrology, nutrient limitation, benthic algae.

Method synopsis

Magnesium hydroxide co-precipitation method using Sargasso Sea water to concentrate low-level soluble reactive phosphorus samples 70-fold prior to colorimetric analysis.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — High-discharge disturbance does not alter the seasonal trajectory of nutrient uptake in a montane stream (2021), Hydrobiologia. Implementations in other papers (listed below) may differ.

1. Analyze water samples for nutrient concentrations
   Analyze NH4+-N using standard fluorometric method, pre-concentrate SRP samples 70-fold using Sargasso Sea water magnesium hydroxide co-precipitation (MAGIC-P) method, analyze using conventional colorimetry on Thermo Scientific GENESYS 10S UV-VIS Spectrophotometer
   Quantities: 30-40 water samples collected per breakthrough curve experimentDuration: Analysis conducted immediately or refrigerated until following dayConditions: Samples kept on ice until lab analysis
   Equipment: Turner Designs Trilogy Fluorometer Model #7200, Thermo Scientific GENESYS 10S UV-VIS Spectrophotometer, MAGIC-P pre-concentration materials

Typical Equipment

• Sargasso Sea water
• magnesium hydroxide

Output Measurements

• concentrated phosphorus samples

Papers Using This Protocol (2)

Species Studied With This Protocol