photoperiodic response curve analysis (Drosophilidae)

Knowledge graph centered on photoperiodic response curve analysis (Drosophilidae) with 21 nodes and 56 connections. Top connected: Atriplex canescens, Lepidoptera, winter survival, Drosophila melanogaster, A. fabae.

Method synopsis

Statistical fitting of dose-response curves to photoperiodic data using log-logistic models to estimate the critical day length (CDL) where 50% of females enter diapause.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Nanda-Hamner Curves Show Huge Latitudinal Variation but No Circadian Components in <i> Drosophila Montana </i> Photoperiodism (2021), Journal of Biological Rhythms. Implementations in other papers (listed below) may differ.

1. Statistical analysis
   Photoperiodic response curves (PPRCs) were estimated using function drm with four-parameter log-logistic model. CDL values were fitted using quasibinomial model with logit link function. Effects of latitude, photoperiod, and experimental status were tested using generalized linear models and quasibinomial models with appropriate corrections for multiple comparisons.
   Quantities: 28 strains analyzed across multiple photoperiodsDuration: Post-experimental analysisConditions: Computational analysis
   Equipment: R software version 3.4.4, R package drc, R package stats, R package ggplot2

Typical Equipment

• R software

Output Measurements

• critical day length
• photoperiodic response parameters

Papers Using This Protocol (1)

Species Studied With This Protocol