Invasive plant removal experiment (Scrophulariaceae)

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Method synopsis

Small-scale removal of invasive plant flowers to test effects on native plant pollination through paired treatment and control plots within experimental blocks. Invasive flowers were removed by clipping inflorescences while control plots maintained intact invasive plants.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Unraveling the ecological and evolutionary impacts of a plant invader on the pollination of a native plant (2021), Biological Invasions doi 10.1007/s10530-021-02457-z. Implementations in other papers (listed below) may differ.

1. Establish experimental blocks
   Placed experimental blocks over continuous L. vulgaris patches. Each block contained two 3m × 3m plots spaced 3 meters apart. Blocks were placed at least 10 meters away from other blocks. Block density was 0.37 P. strictus individuals per m² to resemble average field site density of 0.36 individuals per m².
   Quantities: 7 blocks in 2015, 5 additional blocks in 2016, 5 additional blocks in 2017. Total of 24 plots analyzed across all years.Duration: Established once per year before flowering seasonConditions: Over continuous L. vulgaris patches in subalpine meadows
   Equipment: measuring tape
2. Apply L. vulgaris treatments
   In removal plots, clipped 5 potted or cut inflorescences of P. strictus from the Lupine trail site to remove L. vulgaris flowers. In present plots, left L. vulgaris flowers intact. Both cut and potted inflorescences were brought to field site on day of pollinator observations and set out just prior to beginning observations.
   Quantities: 5 inflorescences per removal plotDuration: Applied immediately before each observation sessionConditions: During flowering season, mid-July through late-August
   Equipment: pruning shears for clipping

Typical Equipment

• measuring tape
• pruning shears for clipping

Output Measurements

• pollinator visitation rate
• seed production
• pollinator community composition

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used