Floral orientation manipulation for fitness measurement (Plantae)

Knowledge graph centered on Floral orientation manipulation for fitness measurement (Plantae) with 71 nodes and 186 connections. Top connected: Hummingbird, Bombus, Ipomopsis, Ligusticum porteri, I. aggregata.

Method synopsis

Experimental manipulation of natural floral orientations combined with pollen supplementation to test effects on seed set and female fitness.

Synthesized from method descriptions across 4 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — The function of floral orientation in bluebells: interactions with pollinators and rain in two species of <i> Mertensia </i> (Boraginaceae) (2019), Journal of Plant Ecology. The protocol was originally introduced by Testing the oviposition cues that drive preference of <i></i>Hylemya<i></i> for different sexes of <i></i>Polemonium foliosissimum<i></i> (2010). Implementations in other papers (listed below) may differ.

1. Manipulate floral orientation for seed set experiments
   Select flowering plants within natural populations and assign each plant to a floral orientation treatment (pendant, horizontal, or upright). Manipulate floral orientation by fitting one metal wire per stem per plant around a bent and looped metal wire inserted into the ground. Apply supplemental pollen to half the plants in each treatment using pollen from two different plants collected at least 3 m from the focal plant.
   Quantities: 30 plants per orientation per species in 2014 (total n = 180), 15 plants per orientation per species in 2015 (total n = 90)Duration: Throughout flowering season to ensure pollen supplementation to all flowersConditions: Natural field conditions
   Equipment: bent and looped metal wire, pollen collection tools

Typical Equipment

• bent and looped metal wire
• pollen collection tools

Output Measurements

• seed set
• proportion of ovules setting seed

Papers Using This Protocol (4)

Species Studied With This Protocol