Persulfate phosphorus digestion

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Method synopsis

Standard colorimetric method for measuring soluble reactive phosphorus and total dissolved phosphorus in water samples using spectrophotometry. Total dissolved phosphorus samples are first digested with K2S2O8 in a pressure cooker.

Synthesized from method descriptions across 7 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — High interspecific variation in nutrient excretion within a guild of closely related caddisfly species (2018), Ecosphere. Implementations in other papers (listed below) may differ.

1. Phosphorus analysis
   Phosphorus was measured using 20 mL filtered water samples collected in glass test tubes. Samples were analyzed as quickly as possible (immediately or the next day) to prevent sample degradation. Total dissolved phosphorus samples were digested with 0.2 g K2S2O8 in a pressure cooker to oxidize all reactive P to PO43-. Both SRP and TDP were measured following a standard colorimetric method using a Thermo Scientific GENESYS 10S UV-VIS Spectrophotometer.
   Quantities: 20 mL samples in glass test tubes, 0.2 g K2S2O8 for digestionDuration: Analysis conducted immediately or next day after collectionConditions: Pressure cooker digestion for TDP analysis, standard colorimetric conditions
   Equipment: glass test tubes, pressure cooker, K2S2O8, Thermo Scientific GENESYS 10S UV-VIS Spectrophotometer

Typical Equipment

• pressure cooker
• K2S2O8
• Thermo Scientific GENESYS 10S UV-VIS Spectrophotometer

Output Measurements

• soluble reactive phosphorus concentration
• total dissolved phosphorus concentration

Papers Using This Protocol (7)

Species Studied With This Protocol

Places Where Used