Multiple-choice feeding preference assay (Limnephilidae)

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Method synopsis

Larvae offered multiple food choices in aquatic containers with instantaneous behavioral sampling over extended time periods to quantify feeding preferences.

Synthesized from method descriptions across 5 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Characterizing the role of phosphorus availability and periphytic algae in the food choice and performance of detritivorous caddisflies (Trichoptera:Limnephilidae) (2021), Freshwater Science. The protocol was originally introduced by Larval cannibalism, time constraints, and adult fitness in caddisflies that inhabit temporary wetlands (2004), Oecologia. Implementations in other papers (listed below) may differ.

1. Conduct growth experiments
   Created 5 replicate chambers per treatment using 36×20 cm clear plastic chambers. Added 15 g wet sedge from single treatment per chamber with 5 caddisflies, covered with shade cloth to inhibit algae growth during experiment
   Quantities: 5 chambers per treatment (20 total), 15 g wet sedge per chamber, 5 individuals per chamberDuration: 9-15 days depending on speciesConditions: Same outdoor shed conditions, chambers covered with shade cloth
   Equipment: 36×20 cm clear plastic chambers, shade cloth

Typical Equipment

• plastic containers
• paper clips
• pond water

Output Measurements

• percent time at food sources
• feeding preference ratios

Papers Using This Protocol (5)

Species Studied With This Protocol

Places Where Used