Chlorophyll-a extraction and fluorometric analysis

Knowledge graph centered on Chlorophyll-a extraction and fluorometric analysis with 71 nodes and 129 connections. Top connected: Bacteria, elevational gradients, aquatic insects, Baetis, Trichoptera.

Method synopsis

Collection of nitrogen samples via syringe filtration and chlorophyll samples via algae collection from submerged rocks, with spectrophotometric analysis normalized by substrate area.

Synthesized from method descriptions across 13 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Does the morphology of beaver ponds alter downstream ecosystems? (2011), Hydrobiologia. Implementations in other papers (listed below) may differ.

1. Benthic algae biomass sampling
   Collected 10 rocks randomly from upstream and downstream of each beaver pond on one date (4-5 August) during summer 2008. Rocks completely extracted (submerged) in 90% MgCO3-buffered ethanol for extraction. Analyzed extracts fluorometrically to estimate chlorophyll a concentration according to EPA Method 445.0. Traced rocks on paper and scanned with LI-COR LI-3100C area meter to estimate two-dimensional area of streambed occupied by each rock. Used rock areas to estimate mean algal biomass per unit area (mg chlorophyll a/m²) in each reach.
   Quantities: 10 rocks per site from upstream and downstream reaches at each beaver pond, total ~240 rocksDuration: Single sampling date August 4-5, 2008Conditions: Summer sampling during 2008 field season
   Equipment: 90% MgCO3-buffered ethanol, fluorometer, LI-COR LI-3100C area meter, paper for rock tracing
2. Pond spillover phytoplankton sampling
   Collected five pond spillover phytoplankton samples at each site on 16-21 July 2008 as potential food resource for suspension-feeding invertebrates. Filtered 1 l of pond spillover water through 0.7 μm pore size Whatman glass microfiber filter. Froze filters and later extracted and analyzed fluorometrically by methods described above. Spillover chlorophyll a concentrations transformed (ln(x)) to meet normality assumptions.
   Quantities: 5 samples per site, 1 liter filtered per sampleDuration: July 16-21, 2008 sampling periodConditions: Summer 2008 field sampling
   Equipment: 0.7 μm Whatman glass microfiber filters, fluorometer

Typical Equipment

• syringe
• glass fiber filter
• opaque sample bottles
• sample bottles
• tinfoil
• spectrophotometer
• ImageJ software

Output Measurements

• nitrogen content
• chlorophyll content

Papers Using This Protocol (13)

Species Studied With This Protocol