QTL mapping using recombinant inbred lines (Brassicaceae)

Knowledge graph centered on QTL mapping using recombinant inbred lines (Brassicaceae) with 67 nodes and 157 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Phenotypic plasticity and adaptive evolution contr, Crested Butte, Boechera stricta.

Method synopsis

Common garden experiments using recombinant inbred lines to partition genetic and environmental contributions to phenotypic variation. RILs are planted in controlled environments to estimate heritability and map quantitative trait loci for flowering time.

Synthesized from method descriptions across 2 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Strong selection genome-wide enhances fitness tradeoffs across environments and episodes of selection (2014), Evolution. Implementations in other papers (listed below) may differ.

1. Generate recombinant inbred lines
   Crossed Montana father (45.7°N, 2460m elevation) with Colorado mother (38.7°N, 2530m elevation) and propagated 172 F6 RILs through self-pollination and single-seed descent over multiple generations
   Quantities: 172 F6 RILs generated from single cross between two parental populationsDuration: Multiple generations through F6Conditions: Laboratory greenhouse conditions for crossing and propagation
   Equipment: controlled pollination equipment

Typical Equipment

• seed trays
• transplanting tools
• plot markers
• data sheets
• hand lenses

Output Measurements

• flowering time
• fitness components
• heritability estimates

Papers Using This Protocol (2)

Species Studied With This Protocol

Places Where Used