Knowledge graph centered on Lepidopteran fat body whole-mount immunofluorescence (Nymphalidae) with 16 nodes and 47 connections. Top connected: Bombus, Bombus terrestris, Lepidoptera, early-life trade-offs, Speyeria mormonia.
A whole-mount immunostaining protocol optimized for visualizing FOXO and mTOR protein localization in butterfly fat body tissue using species-specific antibodies. The protocol involves tissue fixation, permeabilization with detergents, blocking, and fluorescent antibody labeling.
Synthesized from method descriptions across 1 paper using this protocol.
Steps below were extracted from the paper that introduces this protocol — Antibody development to identify components of IIS and mTOR signaling pathways in lepidopteran species, a set of non-model insects (2023), microPublication Biology. Implementations in other papers (listed below) may differ.