Protocols

Standardized measurements of wing length and dried body mass components under stereomicroscope to quantify adult fitness proxies and assess elevation effects on body size.

In-situ measurement of temperature, pH, dissolved oxygen, and barometric pressure at multiple points around each pond during peak photosynthetic activity. Provides standardized water chemistry profile...

Standardized photography protocol using blue light excitation to capture biofluorescence in salamanders under controlled conditions with specific camera settings and positioning.

Calculation of body condition metrics (mass/SVL³) and derived fitness proxies including condition-weighted reproductive opportunities for males and estimated egg production for females based on body s...

Use of ceramic tiles as standardized substrates for measuring benthic algal biomass via chlorophyll-a extraction and fluorometry. Includes grazed vs ungrazed comparisons to assess herbivory effects.