Knowledge graph centered on pollination exclusion experiment with 76 nodes and 288 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee.
Method synopsis
Experimental addition of outcross pollen to flowers to test whether natural pollination limits plant reproductive success, comparing reproductive output between hand-pollinated and naturally pollinated flowers.
Synthesized from method descriptions across 47 papers using this protocol.
Procedure from a recent peer-reviewed implementation
Randomly assigned half the plots to pollen supplementation treatment. Collected supplemental outcross pollen from donor plants outside study plots using forceps, gently rubbed across stigmas of treatment plants. Removed anthers from donor plants and transferred pollen using a standard technique in pollination biology.
Quantities: Supplemental pollen applied to all stigmas on treatment plants, pollen donors usually located further than 5m from focal plantsDuration: Three times per week throughout flowering period of each species (early July for H. fendleri, July-August for D. nuttallianum)Conditions: Applied in morning when anthers contained visible pollen, typically 08:30-10:00h