Longitudinal pustule area measurement

Knowledge graph centered on Longitudinal pustule area measurement with 24 nodes and 77 connections. Top connected: Crested Butte, Artemisia tridentata, Penstemon strictus, Linum lewisii, Cement Creek.

Method synopsis

Repeated photographic measurement of individual fungal pustules over time to quantify replication rates and disease progression. Uses standardized macro photography with consistent framing and lighting.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure as described in the canonical source

Steps below were extracted from the paper that introduces this protocol — Predicting the effects of climate change on the cross? scale epidemiological dynamics of a fungal plant pathogen (2022), Scientific Reports. Implementations in other papers (listed below) may differ.

1. Select and mark focal diseased plants
   Within each transect, designated subset of diseased plants as 'focal diseased plants' for detailed within-host measurements. Selected diseased plants with height approximately 5cm or less initially, obtained at least 25 infected focal plants per site. Tagged plants with colored flagging tape at base of stem for identification.
   Quantities: Minimum 25 focal diseased plants per site, 4 sites totalDuration: Initial selection early in season, plants tracked throughout observation periodConditions: Selected plants too delicate for manipulation until reaching sufficient size
   Equipment: colored flagging tape, metal tags, rulers
2. Photograph and measure pustules on marked stems
   Marked tip of one infected leaf per plant with black ink, then marked preferentially selected leaves with one or few pustules. Photographed each marked leaf within 5cm frame using Canon EOS Rebel T7i DSLR camera fitted with Canon EF-S 35mm f/2.8 Macro IS STM lens and Cannon MR-14EX II Macro Ring Lite flash. Maintained perpendicular shooting angle and consistent photographic settings for pustule boundary identification.
   Quantities: One leaf per plant photographed approximately every 3 daysDuration: Repeated measurements every ~3 days throughout observation periodConditions: Consistent lighting using ring flash, perpendicular angle maintained
   Equipment: Canon EOS Rebel T7i DSLR camera, Canon EF-S 35mm f/2.8 Macro IS STM lens, Canon MR-14EX II Macro Ring Lite, black ink markers

Typical Equipment

• Canon EOS Rebel T7i DSLR camera
• Canon EF-S 35mm f/2.8 Macro IS STM lens
• Canon MR-14EX II Macro Ring Lite
• colored flagging tape
• black ink markers

Output Measurements

• pustule area
• pustule growth rates
• infection intensity

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used