Spore trap dispersal measurement

Knowledge graph centered on Spore trap dispersal measurement with 24 nodes and 77 connections. Top connected: Crested Butte, Artemisia tridentata, Penstemon strictus, Linum lewisii, Cement Creek.

Method synopsis

Systematic deployment of adhesive spore traps at standardized distances and directions around infected plants to quantify spore dispersal patterns and relate to environmental conditions.

Synthesized from method descriptions across 1 paper using this protocol.

Procedure as described in the canonical source

Steps below were extracted from the paper that introduces this protocol — Predicting the effects of climate change on the cross? scale epidemiological dynamics of a fungal plant pathogen (2022), Scientific Reports. Implementations in other papers (listed below) may differ.

1. Deploy spore traps around diseased plants
   Deployed spore traps in arrays around subset of focal diseased plants. Traps consisted of 2cm² section of Scotch Permanent Clear Mounting Tape (Part #: M117) affixed to plastic backing with double sided tape. Secured into ground with 5cm nails. Placed traps at distances of 5cm, 25cm, 50cm, and 100cm in four directions corresponding to transect axes.
   Quantities: 16 traps per focal plant (4 distances × 4 directions), subset of diseased plants trappedDuration: Traps left in field for approximately one week then collectedConditions: Traps placed as close to ground as possible to minimize spores from other plants
   Equipment: Scotch Permanent Clear Mounting Tape Part #M117, plastic backing, double sided tape, 5cm nails, measuring tapes
2. Count spores on trap surfaces
   Transferred sections of mounting tape to microscope slides and sealed with clear packing tape. Used light microscope to count spores in 0.8cm² area for most spore traps, counting minimum area of 0.1cm² for all traps including those with >10,000 spores per cm². Identified spores visually using slide prepared with reference spores.
   Quantities: 0.8cm² counting area for most traps, 0.1cm² minimum for high-density trapsDuration: Microscopy conducted after field season completionConditions: Laboratory conditions using prepared microscope slides
   Equipment: light microscope, microscope slides, clear packing tape, reference spore slides

Typical Equipment

• Scotch Permanent Clear Mounting Tape Part #M117
• light microscope
• microscope slides
• plastic backing
• double sided tape

Output Measurements

• spore deposition rates
• dispersal distances
• spore counts

Papers Using This Protocol (1)

Species Studied With This Protocol

Places Where Used