Knowledge graph centered on Multi-site flax rust epidemic monitoring with 70 nodes and 153 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Boechera stricta, Artemisia tridentata, Climate Change Impacts.
Method synopsis
Weekly monitoring of flax rust disease prevalence across multiple wild flax populations to track epidemic progression and relate it to local environmental conditions over multiple growing seasons.
Synthesized from method descriptions across 2 papers using this protocol.
Four 200-m² plots were established spanning a large elevational gradient in the Rocky Mountains, with each plot containing naturally occurring flax populations
Quantities: Four plots of 200 m² eachDuration: Established for five growing seasons (2019-2023)Conditions: Rocky Mountain elevational gradient
Equipment: plot markers
Record diseased plants at season start
At the beginning of each season, the number of diseased plants was recorded towards the beginning of the season
Quantities: All diseased plants in each 200-m² plotDuration: Beginning of each growing seasonConditions: Early growing season
Equipment: field recording materials
Conduct weekly disease surveys
Every week, previously non-diseased plants were checked for new infections to track population-level epidemiological data and within-host disease progression data for several focal plants (~10% of each plant population)
Quantities: ~10% of each plant population as focal plantsDuration: Weekly throughout growing season (mid-June to early-August)Conditions: Growing season conditions
Weekly disease prevalence was calculated using the formula: Prevalence = (It-1 + Jt - γt × It-1) / N, where I represents cumulative number of infected plants, J represents weekly new infections, gamma represents weekly disease depletion rate, and N is total population size
Quantities: All plants in each populationDuration: Weekly calculations throughout each seasonConditions: Post-field data collection