Protocols

Microscopic assessment of arbuscular mycorrhizal fungal colonization and other fungal colonization of plant roots using crosshair intercept method and grid line-intercept method for soil fungi.

Surface sterilization of plant tissues followed by DNA extraction and high-throughput sequencing of the ITS region to characterize fungal endophyte communities. Community composition and diversity met...

Hierarchical Bayesian models predicting vital rates from individual and neighborhood characteristics, with population-level random effects and model selection via WAIC.

Field identification of lichen species using chemical spot tests with potassium hydroxide, morphological examination with hand lens, and comparison to field guide keys.

Extraction of plant metabolites using methanol/acetonitrile/water solvent followed by liquid chromatography-mass spectrometry analysis. Characterizes plant metabolomic profiles for correlation with fu...