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17 protocols
Microscopic assessment of arbuscular mycorrhizal fungal colonization and other fungal colonization of plant roots using crosshair intercept method and grid line-intercept method for soil fungi.
Systematic monitoring of plant disease dynamics across elevation gradient to capture environmental variation effects. Involves establishment of standardized transects with complete plant population ma...
Surface sterilization of plant tissues followed by DNA extraction and high-throughput sequencing of the ITS region to characterize fungal endophyte communities. Community composition and diversity met...
29-year experimental warming using overhead infrared radiators to increase soil temperature by 2°C in subalpine grassland plots. Heat flux of 22 W/m² applied continuously from 1991-2019.
Collection of soil inocula from resident (high elevation) and novel (low elevation) sites across elevation gradients in mountain ecosystems, with subsequent sterilization treatments to isolate microbi...