Protocols

Controlled choice experiments measuring butterfly oviposition preference between plant species using matched plant materials in screen cages. Preference quantified as proportion of eggs laid on test p...

Transplant butterflies between high and low elevation sites to quantify differences in flight initiation timing and thermal sensitivity across elevation gradients using caged trials.

A controlled feeding experiment where individual first instar larvae are offered different plant species and their gut content accumulation is monitored over time through categorical and quantitative ...

Multi-generation breeding program with controlled matings to estimate heritability of preference traits using pedigree-based models accounting for ZW sex determination system.

Daily forced flight protocol involving 3 × 4 minute flight bouts with rest periods, stimulated by gentle brushing, to experimentally increase energy expenditure in butterflies. Control group remains i...