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Rocky Mountain Biological Laboratory · Gothic, Colorado · founded 1928. A non-profit field station for long-term ecological research.

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P.O. Box 519 Crested Butte, CO 81224 · (970) 349-7231 · RMBL is a Colorado non-profit organization with IRS 501(c)(3) status. RMBL is an equal opportunity service provider and employer and operates under permit from the USDA Forest Service, Gunnison National Forest. Support for the Knowledge Commons provided by the Clark Family Foundation.

Filtered by neighborhood:Alpine Plant-Pollinator Interactions and Bee Foraging Ecology Clear filter

Protocols

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9 protocols

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Helianthella flower removal experiment (Asteraceae)

Experimental removal of flower buds to simulate frost damage effects, allowing comparison of microclimate conditions between plots with and without flower displays in subalpine meadow.

phenological manipulation9 papersmeasuring tape, plot markers...

experimentalstandardized

Reciprocal egg-transfer experiment for bee larval survival (Megachilidae)

Laboratory experiment transferring bee eggs between different pollen provision types to test effects on larval survival. Eggs moved between Taraxacum and native Asteraceae provisions in a balanced rec...

controlled manipulation7 papersscalpel, microsurgical instruments...

experimentalstandardized

Factorial phenology-water manipulation of potted wildflowers

A factorial experiment manipulating both flowering phenology (by moving plants between elevations) and water availability (through differential watering regimes) using potted native plants in field ar...

climate manipulation5 papers2-gallon plastic pots, mesh shade-shelter...

experimental

Floral orientation manipulation for fitness measurement (Plantae)

Experimental manipulation of natural floral orientations combined with pollen supplementation to test effects on seed set and female fitness.

reproductive4 papersbent and looped metal wire, pollen collection tools

experimentalstandardized

Butterfly-mediated microbial dispersal experiment (Nymphalidae)

Controlled experiment using marker bacterium to track microbial transfer from inoculated donor flowers to recipient flowers via butterfly foraging behavior.

microbial transmission3 papersfloral picks, micropipette...

experimental

Single-species pollinator removal experiment

Controlled removal of the most abundant Bombus species from plots using targeted aerial netting during 4-hour manipulation periods, compared to 4-hour control periods with no removal. Pollinator exclu...

community manipulation3 papersaerial nets, specimen vials...

experimental

pollinator exclusion

Application of fine-mesh bags over flowers to prevent pollinator access while maintaining airflow and light penetration. Uses Del Star polyethylene bags with 0.5mm pores applied to approximately 50% o...

pollination manipulation3 papersDel Star polyethylene bags, 0.5mm pore mesh

experimental

Critical thermal maximum (CTmax)

Controlled heating and cooling assays to determine critical thermal maximum (CTmax) and minimum (CTmin) temperatures of bumblebees under field conditions. Includes environmental optimization trials to...

thermal physiology2 papersStyrofoam boxes, aluminum block...

experimental

reciprocal transplant experiment (Animalia)

Experimental transplantation of completed mason bee nests from low to high elevation sites to test effects of season length and climate on offspring development. Uses trap-nest monitoring followed by ...

elevational transplant2 paperswooden blocks (2"x6"), hollow paper tubes...