Protocols

Multiple candidate models incorporating different combinations of climate predictors were fitted and ranked using Akaike Information Criterion to select optimal models for demographic functions.

A standardized protocol for preparing plant leaves through chemical clearing and measuring vein density using microscopy and stochastic line-intersection analysis. Leaves are dried, cleared with sodiu...

Calculation of species area of occupancy (AOO) using filtered GBIF occurrence records and standardized grid-based methods following IUCN guidelines for conservation assessment.

Genotyping using a panel of 384 candidate-gene single nucleotide polymorphisms putatively associated with adaptive traits.

Statistical validation approach for model selection and parameter optimization to ensure robust performance.