Fuchsin jelly pollen extraction

Knowledge graph centered on Fuchsin jelly pollen extraction with 72 nodes and 241 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee.

Method synopsis

Quantitative analysis of pollen grain composition in bee provisions using compound microscopy. Pollen samples stained with fuchsin and examined at 100× magnification with systematic transect counting to determine taxonomic composition.

Synthesized from method descriptions across 14 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation (2025), Applications in Plant Sciences. The protocol was originally introduced by Comparing pollen distribution on pollinators to floral community composition in the East River Valley (2021). Implementations in other papers (listed below) may differ.

1. Extract DNA from pollen samples
   Use modified CTAB method with sodium dodecyl sulfate (SDS) extraction buffer containing 350mM Tris-HCl, 50mM EDTA, 50mM NaCl, 10% SDS v/v, pH 7.5. Clean DNA extracts using 2:1 v/v Sera-Mag beads, elute in 0.5x Tris-EDTA. Quantify DNA using Qubit fluorometer.
   Quantities: 54 samples successfully sequenced from 64 collectedDuration: Not specifiedConditions: Laboratory conditions
   Equipment: CTAB reagents, Sera-Mag beads, Tris-EDTA, Qubit fluorometer
2. Prepare sequencing libraries
   Use NEBNext Ultra II FS DNA Library Prep Kit for Illumina. Fragment at half volume, perform PCR enrichment with Angiosperms353 probe kit V.4. Use recommended volumes of 80% v/v ethanol washes and SPRI beads for cleanup. Sequence using Illumina MiSeq with 150-bp paired-end reads.
   Quantities: 500 ng cleaned DNA input, 80% v/v ethanol wash volumesDuration: Not specifiedConditions: Laboratory conditions following manufacturer's protocol
   Equipment: NEBNext Ultra II FS DNA Library Prep Kit, Angiosperms353 probe kit V.4, SPRI beads, Illumina MiSeq

Typical Equipment

• compound microscope
• fuchsin stain
• microscope slides

Output Measurements

• pollen grain counts
• taxonomic composition
• Taraxacum usage proportion

Papers Using This Protocol (14)

Species Studied With This Protocol

Places Where Used