browse damage assessment

Knowledge graph centered on browse damage assessment with 74 nodes and 161 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Yellow-fronted Bumble Bee.

Method synopsis

Twice weekly censuses recording plant vital status, reproductive phenology, and fitness components including flower and fruit production. Weekly assessments of herbivore damage quantify leaf area removal.

Synthesized from method descriptions across 31 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Relaxation of the leaf economics spectrum within and across quaking aspen Populus tremuloides genotypes (2025), Oikos. The protocol was originally introduced by Response of plant pathogens and herbivores to a warming experiment (2004), Ecology. Implementations in other papers (listed below) may differ.

1. Assess leaf herbivory damage
   Cut two 30-50 cm large branches per tree (south and north facing) and counted total number of leaves and number of herbivory-damaged leaves. From herbivory-damaged batch of leaves, randomly selected five leaves, removed petiole and scanned at 300 dpi using CanoScan LiDE220 color image scanner. Herbivory damage was calculated as percentage difference between herbivory and reconstructed leaf area.
   Quantities: 2 branches per tree, 5 randomly selected damaged leaves per tree for scanningDuration: End of growing season (end of July to mid-August 2017)Conditions: After leaf collection for trait measurements
   Equipment: CanoScan LiDE220 color image scanner (Canon), Adobe Photoshop, MATLAB-based image analysis code

Typical Equipment

• calipers
• measuring tape
• data sheets

Output Measurements

• survival probability
• reproductive status
• fecundity
• herbivore damage
• plant size

Papers Using This Protocol (31)

Species Studied With This Protocol

Places Where Used