reflectance spectrophotometry

Knowledge graph centered on reflectance spectrophotometry with 73 nodes and 247 connections. Top connected: Rocky Mountain Biological Laboratory, Gothic, Two-form Bumble Bee, Bumble Bees, Buff-tailed Bumble Bee.

Method synopsis

Standardized measurement of floral reflectance spectra using fiber optic probe positioned at fixed distance and angle from flower surface. Spectra collected across 300-700 nm range for input into visual models.

Synthesized from method descriptions across 11 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Birds Perceive More Intraspecific Color Variation in Bird-Pollinated Than Bee-Pollinated Flowers (2020), Frontiers in Plant Science. doi 10.3389/fpls.2020.590347. Implementations in other papers (listed below) may differ.

1. Spectrophotometry measurements
   Took spectral readings within 12 hours of flower collection. For species with multiple human-distinguishable color patches, chose patch representing greatest surface area. Mounted individual petal or bract on cellophane tape and affixed to bottom of probe-holder block. Took measurements spanning 300-700 nm using bifurcated coaxial fiber optic reflectance probe held at 45° to flower surface, 8.0 mm from surface, with petal tip facing away from probe.
   Quantities: One spectrum per flower, spectral range 300-700 nm, probe distance 8.0 mm, 45° angleDuration: Within 12 hours of flower collection, 10 ms integration timeConditions: Measurements taken with fiber optic probe held at standardized position
   Equipment: Avantes model 2048 spectrometer, bifurcated coaxial fiber optic reflectance probe (Avantes FCR-7uv200-2-ME), AvaLight-XE xenon light source, diffuse white PTFE tile (Avantes WS-2)

Typical Equipment

• Avantes model 2048 spectrometer
• bifurcated coaxial fiber optic reflectance probe (Avantes FCR-7uv200-2-ME)
• AvaLight-XE xenon light source
• diffuse white PTFE tile (Avantes WS-2)

Output Measurements

• reflectance spectra
• spectral data 300-700 nm

Papers Using This Protocol (11)

Species Studied With This Protocol

Places Where Used