Gynodioecious plant pollen receipt census

Knowledge graph centered on Gynodioecious plant pollen receipt census with 63 nodes and 127 connections. Top connected: Delphinium, Spruce, Atriplex canescens, elevational gradients, Delphinium nuttallianum.

Method synopsis

Field census method to quantify pollen receipt patterns in populations of gynodioecious plants with varying sex ratios and floral densities. Involves systematic counting of flowers by sex and measurement of pollen grains received per flower.

Synthesized from method descriptions across 6 papers using this protocol.

Procedure from a recent peer-reviewed implementation

Steps below were extracted from the most recent peer-reviewed implementation of this protocol in the corpus — Hydrologic connectivity shapes watershed response to climate variability from local to global scales (2025). Implementations in other papers (listed below) may differ.

1. Population selection and characterization
   Four spatially discrete populations were selected near Rocky Mountain Biological Laboratory: Pumphouse (PH), Aspen, Research Meadow (RM), and Virginia (VA). Each population was characterized for habitat type, plant density, and sex ratios across different areas within each site.
   Quantities: Four populations spanning a range of habitats, plant densities and sex ratiosDuration: Initial site characterization, then ongoing throughout study periodConditions: Subalpine region near Gothic, Colorado at 2900m elevation, flowering season from late June to early August
   Equipment: measuring tape for plot establishment
2. Weekly flower tagging
   At the beginning of each week, flowers were tagged with small paper jewelry tags tied below the flower for later collection. Two categories were tagged: flowers for stigma pollen collection and flowers for seed set assessment. Eligible flowers included open flowers that had not yet lost their anthers and floral buds about to open.
   Quantities: 60-90 flowers tagged for stigma collection, 20-30 flowers tagged for seed set per population per weekDuration: Weekly from June 26 through July 30, 2001Conditions: During flowering season, tags placed at beginning of each week
   Equipment: small paper jewelry tags, ties or string
3. Local mating environment assessment
   At the time of floral tagging, the number of open hermaphrodite flowers and female flowers within a 0.5 m radius were counted to determine the local mating environment of each target flower.

Typical Equipment

• field counting methods
• pollen counting methods
• calculation methods

Output Measurements

• hermaphrodite flower counts
• female flower counts
• pollen grain receipt per flower
• floral density
• sex ratio

Papers Using This Protocol (6)

Species Studied With This Protocol