Protocols

Genomic sequencing and demographic modeling using GADMA2 to estimate historical gene flow patterns and migration rates between populations across elevational gradients.

Construction of Integral Projection Models using vital rate data to project population persistence under different climate scenarios and estimate stochastic population growth rates.

Sanger sequencing of BCMA3 alleles from natural accessions combined with Nanopore long-read genome assembly to identify structural genetic variants affecting glucosinolate biosynthesis.

Conditional Neutrality-Antagonistic Pleiotropy analysis estimates realized selection coefficients and uses permutation tests to identify genome-wide patterns of fitness trade-offs across environments ...

Chromosome-specific BAC contigs of Arabidopsis thaliana were used as painting probes to analyze mitotic and meiotic chromosomes of C. cordifolia. Fluorescent signals were detected and analyzed to dete...