Protocols

A large-scale reciprocal transplant experiment across five elevational gardens with factorial snow and temperature manipulations to test local adaptation and evolutionary rescue potential under climat...

Twice weekly censuses recording plant vital status, reproductive phenology, and fitness components including flower and fruit production. Weekly assessments of herbivore damage quantify leaf area remo...

A factorial field experiment manipulating snowmelt timing using black shade cloth and summer precipitation using rainout shelters in a split-plot design to simulate climate change effects.

Weekly monitoring of plant phenology, morphological traits, and density across an elevational gradient to assess phenotypic plasticity in response to environmental conditions. Uses standardized phenol...

Greenhouse factorial experiment manipulating water availability (3 levels) and nutrient supply (2 levels) to test resource limitation effects on fitness components. Plants grown from field-collected s...

Genomic sequencing and demographic modeling using GADMA2 to estimate historical gene flow patterns and migration rates between populations across elevational gradients.

Systematic assessment of flower color variation across environmental gradients using standardized color categories and plot-based sampling. Combines phenotypic scoring with environmental variable meas...

Construction of Integral Projection Models using vital rate data to project population persistence under different climate scenarios and estimate stochastic population growth rates.

Standardized greenhouse cultivation of field-collected plant accessions under controlled temperature, light, and nutrition regimes for phenotype characterization.

Acrylic imprints of leaf surfaces are made on glass slides and analyzed under compound microscope to count stomata and measure their diameters.

Sanger sequencing of BCMA3 alleles from natural accessions combined with Nanopore long-read genome assembly to identify structural genetic variants affecting glucosinolate biosynthesis.

Conditional Neutrality-Antagonistic Pleiotropy analysis estimates realized selection coefficients and uses permutation tests to identify genome-wide patterns of fitness trade-offs across environments ...

Chromosome-specific BAC contigs of Arabidopsis thaliana were used as painting probes to analyze mitotic and meiotic chromosomes of C. cordifolia. Fluorescent signals were detected and analyzed to dete...

Hand-pollination experiment comparing fitness of selfed vs. outcrossed offspring by controlling pollination source and measuring resulting fruit characteristics. Uses reciprocal crosses between geneti...

A factorial field experiment manipulating herbivore abundance and water availability using cages with controlled grasshopper access and supplemental watering or rain-out shelters. Plants from multiple...

Comprehensive measurement of multiple fitness components including survival, reproductive success, fecundity, and growth rates to assess life history trade-offs under resource stress.

Multi-environment QTL analysis using recombinant inbred lines tested across vernalization, photoperiod and temperature treatments to identify genomic regions controlling flowering time. Combines contr...

Common garden experiments using recombinant inbred lines to partition genetic and environmental contributions to phenotypic variation. RILs are planted in controlled environments to estimate heritabil...

Estimation of genetic parameters including heritability and genetic variance-covariance matrices using generalized linear mixed models with Bayesian MCMC methods.

Controlled experiment where subjects are not given choices, used to test responses under specific conditions without alternatives.