Protocols

A large-scale reciprocal transplant experiment across five elevational gardens with factorial snow and temperature manipulations to test local adaptation and evolutionary rescue potential under climat...

A factorial field experiment manipulating snowmelt timing using black shade cloth and summer precipitation using rainout shelters in a split-plot design to simulate climate change effects.

Greenhouse factorial experiment manipulating water availability (3 levels) and nutrient supply (2 levels) to test resource limitation effects on fitness components. Plants grown from field-collected s...

Standardized greenhouse cultivation of field-collected plant accessions under controlled temperature, light, and nutrition regimes for phenotype characterization.

Hand-pollination experiment comparing fitness of selfed vs. outcrossed offspring by controlling pollination source and measuring resulting fruit characteristics. Uses reciprocal crosses between geneti...